365 papers
This study demonstrates that DNA ligation-mediated multiple displacement amplification (DLMDA) effectively boosts DNA yield and preserves global copy-number alteration patterns in aged FFPE prostate tumor samples for whole-genome sequencing, although it significantly reduces the sensitivity for detecting specific amplifications and deletions.
This study systematically evaluates the 12-month stability of 44 RNA nucleoside standards stored at -80°C and -20°C, identifying specific degradation patterns and purity issues to establish practical guidelines for storage, quality control, and preparation that enhance the accuracy and reproducibility of LC-MS-based RNA modification analysis.
This study utilizes direct RNA sequencing to reveal that distinct epitranscriptomic landscapes, particularly enhanced uridine modifications within GUUUU motifs in pro-inflammatory vascular smooth muscle cells, constitute a novel regulatory layer governing post-transcriptional mechanisms critical to atherosclerosis progression.
This study demonstrates that cytosolic and ER-associated ribosomes in human cells share highly similar rRNA 2'-O-methylation landscapes, indicating that this modification does not establish a compartment-specific signature or drive ribosome localization to the endoplasmic reticulum.
This study demonstrates that KDM2B acts as a cell type-dependent regulator of HIF-1 expression and activity through its JmjC and CxxC domains, thereby facilitating RNA Pol II recruitment and supporting cellular proliferation under hypoxic conditions.
This study reveals that rapidly evolving aphid gall effector proteins, known as bicycle proteins, adopt diverse saposin-like folds that likely evolved from ancestral disulfide-bonded structures to target various plant mechanisms and evade immune surveillance.
This study reveals that Zika virus infection reshapes the host epitranscriptome by inducing noncanonical alternative polyadenylation to generate new RNA isoforms that are preferentially targeted by METTL3, thereby driving isoform-specific m6A remodeling and altering immune-related signaling pathways.
This study quantifies barcode crosstalk in Oxford Nanopore multiplex sequencing, demonstrating that while updated protocols significantly reduce misassignment compared to older versions, pooling barcoded samples only after adapter ligation (an in-house protocol) offers the most effective mitigation for low-input DNA samples.
This study reveals that the chromatin remodelling complex NuRD facilitates a two-phase "enhancer resetting" process in mouse ES cells, enabling rapid and precise transcriptional responses to ERK signalling by releasing paused RNA Polymerase II and re-establishing a stable, context-appropriate chromatin state.
This paper expands and optimizes the auxin-inducible degron 2 (AID2) system for *Candida* research by introducing versatile template vectors, streamlined CRISPR/Cas9 strategies, and validated protocols that enable efficient protein depletion in prototrophic strains, including clinical isolates and *Candida auris*.
This paper presents a 3D printed hybrid paper-polymer vertical flow device that utilizes a programmable DNA logic gate based on toehold-mediated strand displacement to achieve highly sensitive, label-free, and specific fluorescence detection of platelet-derived growth factor BB (PDGF-BB) at picomolar levels for early-onset preeclampsia screening.
This study utilizes molecular simulations to compare the binding interactions and conformational effects of the natural ligand SAM, the non-functional analog SAH, and a potential binder JS4 on the SAM-I riboswitch, providing critical insights for designing effective RNA-targeting antibiotics.
Vitamin D3 alleviates nitrogen mustard-induced skin damage by remodeling the gut microbiota to enrich *Akkermansia muciniphila* and boost butyrate production, a process mediated through the intestinal VDR-α-defensin signaling pathway.
This study identifies a mutant miR-3260 with a loop structure deletion in a translucent-skinned silkworm strain, revealing that while the wild-type miRNA targets juvenile hormone-related genes, the loop mutation prevents Dicer-mediated cleavage yet fails to produce the expected phenotypic effects.
This study identifies and characterizes nine Heavy metal ATPase (VrHMA) genes in mung bean, revealing their structural diversity and specific upregulation patterns under Zn, Cd, and Cu stress, particularly highlighting VrHMA5's role in vascular transport and the family's potential for improving heavy metal tolerance and micronutrient homeostasis.
This study reveals that ribosome stalling, which frequently occurs as chloroplast transit peptides emerge from the ribosome exit tunnel, serves as a critical regulatory mechanism that enhances the efficient targeting of nuclear-encoded proteins to chloroplasts in *Arabidopsis thaliana*.
This study identifies that Ebola virus replication and transcription are driven by host cell remodeling mediated by cyclin-dependent kinases (particularly CDK2), which can be effectively inhibited to block viral processes, highlighting these kinases as promising antiviral targets.
This study integrates multi-omics data and functional validation in human dopaminergic neurons to identify Parkinson's disease-associated regulatory variants that modulate SCARB2 and BAG3 expression by altering transcription factor binding and chromatin accessibility, thereby elucidating potential mechanisms underlying neuronal degeneration.
This study characterizes the zebrafish mitochondrial transcriptome using long-read sequencing to identify a novel, ubiquitously expressed polyadenylated lncRNA (lncOriL) and specific mRNA features that are conserved across teleost fish, pigs, and humans.
The tumour suppressor RBM5 enforces apoptotic alternative splicing by acting as a dual-action gatekeeper that physically stalls spliceosome progression while simultaneously activating the DHX15 helicase to unwind the branch helix.